The second leg will take place at the Stadio Artemio Franchi next Thursday night, February 26.
FLORENCE, ITALY – FEBRUARY 7: Head coach Paolo Vanoli, manager of ACF Fiorentina, reacts during the Serie A match between ACF Fiorentina and Torino FC at Artemio Franchi on February 7, 2026 in Florence, Italy. (Photo by Gabriele Maltinti/Getty Images)
La Viola make no secret of their goal of avoiding relegation to Serie A, despite having done so well in the Conference League in recent years.
Moise Kean and Dodo are not fully fit and have also been left behind, plus Marco Brescianini, Oliver Christensen and Daniele Rugani are not on the UEFA squad list so are not eligible.
FLORENCE, ITALY – JANUARY 27: Jack Harrison acxf watches during the Coppa Italia match between ACF Fiorentina and Como 1907 at Stadio Artemio Franchi on January 27, 2026 in Florence, Italy. (Photo by Gabriele Maltinti/Getty Images)
It means goalkeeper Lezzerini will make his second UEFA appearance for Fiorentina, the first being a 3-2 defeat against PAOK Thessaloniki in November 2016.
Piccoli leads the attack, supported by January signings Fabbian and Harrison.
Jagiellonia: Abramowicz; Wojtuszek, Bernardo Vital, Pelmard, Wdowik; Pozo, Flach, B Mazurek, Jozwiak; Bazdar, Imaz
Jagiellonia Bank: Damasiewicz, Kobayashi, Drachal, Rallis, Sylla, Krasiewicz, Kozlowski
Fiorentina: Lezzerini; Fortini, Comuzzo, Ranieri, Gosens; mandrake; Harrison, Ndour, Fabbian, Fazzini; Piccoli
Fiorentina bench: Leonardelli, Magalotti, Pongracic, Fagioli, Kouadio, Braschi, Balbo, Bonanno, Deli, Parisi, Sadotti, Bertolini